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The picturesque limestone karsts across the Sino-Vietnamese border are renowned biodiversity hotspot, distinguished for extremely high endemism of calciphilous plants restricted to caves and cave-like microhabitats that have functioned as biological refugia on the otherwise harsh habitats. To understand evolutionary mechanisms underlying the splendid limestone flora, dated phylogeny is reconstructed for Asian Begonia , a species-rich genus on limestone substrates represented by no less than 60 species in southern China, using DNA sequences of nrITS and chloroplast rpL16 intron.
The sampling includes 94 Begonia species encompassing most major Asian clades with a special emphasized on Chinese species. Except for two tuberous deciduous species and a species with upright stems, a majority of Sino-Vietnamese limestone Begonia SVLB , including sect. Coelocentrum 19 species sampled and five species of sect.
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